Please, inform me, if accessibility of virtual slide is problematic. Max resolution 20x. Thank you for patience. "Ordinary" slides will be apended soon.

By morphology without immunohistochemistry I think that this is a case of mostly diffuse follicular lymphoma ( I don't understand CD 56 positivity???)

Some IH stains are placed in. Appologies for mistyping.

Thank you for the nice stains. Would you agree with a mostly diffuse FL or do you favour multilobulated DLBCL with some inflammatory background ? This is hard to decide: In my view the tumor cells represent cytologically Centroblasts and centrocytes ( favouring FL ) but this is not easy to decide at this resolution.

Thank you. The caliber of the cells are large (esp. in CD20)("large centrocytes" and centroblasts), but low proliferative activity favors FL. This case and some last cases presented in iPath were CD23+: I've checked controls, unfortunatelly techno process is not a reason for CD23 false positivity. It's unusual for me such CD23+ in large cells, esp thinking about FL. In DLBCL version maybe such positivity may represent involvement of primary mediastinal LBCL (CD30 pending; I have no add stains for it)?  
 
In future I will enhance magnification of scans up to 40x. Without "virtual slide" sometimes very difficult to demonstrate architectural changes. The another problem of "static" digital's is colocalisation changes esp. in IH stains (taking pictures from the same region). In serial cuts the region architecture is changing and consultant's review became more and more difficult.  
 
A lot of thanks for continuing patience working in such complicated condition.

Again I could not open the virtual slide. I manged only in one orevious case, and there I was not happy with the resolution. Could you please at least in this initial period add some oldfashioned digital images of H&E stained sections, overview magnnifications 20 and 40. Thanks for your understanding.

Dear Prof. Nina, Appologies for inconvenience. I will try always append ordinary pictures and sinchronise with virtual slide. Give me some time, please.

PHOTOS are placed in. Loading. Pleas, refresh the webpage after some minutes.

I agree with the previous comments. My impression is that this is a follicular lymphoma, but it is difficult to be sure of the overall architecture. Regarding CD23, I have noticed that this group expects follicular and large B-cell lymphoma to be CD23-negative by immunohistochemistry, but B-cell lymphomas (with the notable exception of mantle cell lymphoma) are often CD23-positive by flow cytometry. Maybe this is due to different sensitivity of the methods.

Thanx. The question is: the compatibility of CALIBER/CYTOLOGY of cells with difusse pattern (grade II FCC lymphoma).

My impression is follicular lymphoma, grade II. I think there is some evidence of follicular architecture, and smaller cells predominate.

I also agree with the diagnosis of follicular lymphoma. The folliclar pattern is nicely demonstrated on one of the smaller lymohnodes on both images of the whole slide of CD20 and CD3 stained sections,whereas the large node shows a diffuse pattern.  
I also support Dianes comment that FL can be either CD23+/or - on immunohistochemistry. The positivity for CD23 oon flow is probably due to the more sensitive method.  
 
Ugnius thanks for the uploaded beautiful conventional images of the H&E sections. My MacBookPro2.2 cannot mange the wonders of modern technology, such as your scanned slides. I cannot open it. It might be a Freudian Error, because as you know I am not sure for whom the sale of these expensive devices if of higher benefit for you or the company.