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Dx of bacteremia on peripheral smear (3405)
Dx of bacteremia on peripheral smear closed
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munafdesai
2007-07-08 06:09
INCTR - EBMWG Hematopathology Online
Has anybody seen/reported bacteria on peripheral smear? I am posting the images of a peripheral smear showing intracellular & extracellular bacterial organisms. The sample is of a 23 yrs old male patient came to ER with high grade fever. The total WBC count was 6500/cumm, 1-2% band cells were there.  
Please give your comments.  
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metz
2007-07-09 06:56
The films show diplococci within the neutrophils. This is a rare but well-recognized phenomenon. It occurs mainly in patients with overwhelming septicaemia, or in hyposplenic or immunosuppressed patients. It is usually fatal.  
 
This finding should always be correlated with the clinical  
picture, for it is possible that the phenomenon may occur in vitro in contaminated old blood specimens that have been left at room temperature.
Devenish
2007-07-09 15:14
Yes I have certainly seen bacteria in the peripheral blood smear. I am interested to know if you did a Gram Stain.  
Were they Gram positive or Gram negative diplcocci?  
 
Did the patient survive?
munafdesai
2007-07-09 15:37
Thanks Dr Metz & Dr Devenish for the comments.  
The clinical impression was of septic shock. Pigmented spots all over body. PT was 17.9 sec with INR: 1.5, aPTT was 76 sec with ratio of 2.5, D-dimer was 5.4 mg/dl(normal < 3 mg/dl). Possibility of contamination has also been ruled out by checking second sample after 6 hrs interval, which also showed intracellular bacteria. Gram stain is in favour of gram negative diplococci. However, final report will be given on blood culture growth which will be ready by tomorrow morning. I am uploading Gram stain images. The patient is survived.  
 
Thanks  
hurwitz
2007-08-26 18:53
Thanks Dr.Desai, for this very instructive case. we can conclude it with the diagnosis:  
Peripheral blood smear with gram negative diplococci in a patient with septisemia.
diane.c.farhi
2007-09-19 18:06
I am a new member of the group and would like to begin contributing to the case evaluations. I realize that this case is closed, and I agree with the diagnosis of intracellular diplococci, which has certainly been reported. The clinical history sounds like meningococcemia; a less likely possibility (in the USA) is disseminated gonococccus. Are there any blood culture results? Antibiotic therapy? Thank you for allowing me to contribute.
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Last modified: 2007-07-08 06:09:08